Translational_Unit

Part:BBa_K152003:Design

Designed by: David Johnston Monje   Group: iGEM08_Guelph   (2008-10-30)


CrtY + RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We needed to use site directed mutagenesis to add the SpeI site after the stop codon.


Source

The plasmid pUC-EUY which was sent to us by Ethan Johnson on iGEM Minnesota team timebomb.

References